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Catalytic detection principle for HPLC: Determination of enantiomeric iodinated thyronines in blood serum
Summary
Type:
HPLC
Application #:
106330
Detection:
visible absorption, 600nm
Conditions:
eluent: methanol - water (67:33) + 0.05% methanesulphonic acid
flow rate 0.5ml/min
pressure 1100psi
Authors:
Lankmayr EP
Source:
J Chromatogr 224 (1981) 239 - 248
Details
Legend: Fig. 5. Chromatograms of iodinated thyronines with catalytic detection, A) determination of total T4, injection volume 50µl ethanolic serum extract, 1. free halide ions, 2. 10ng T3 (int. std.), 3. 8
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